Colorectal cancer (CRC), the second most diagnosed cancer in Malta, is highly curable through various treatment strategies, if found at an early stage. However, one of the obstacles typically encountered throughout the course of treatment with chemotherapy is the development of drug resistance. A tumour can be intrinsically drug-resistant, meaning resistant from the start of therapy, or acquires resistance throughout the course of treatment. Cancer cells can develop resistance through various mechanisms. One of the ways by which cancer cells escape the toxic effects of the chemotherapy drugs is by altering proteins essential for cell survival. Similar to how one can modify a car to improve its performance, a cancer cell can chemically modify proteins within it to work differently, based on the conditions it experiences. One such chemical modification involves the addition of methyl groups, through a process known as protein methylation. It is important to identify and validate molecules involved in such chemoresistance mechanisms in order to improve treatment strategies. Such molecules, called biomarkers, can be followed throughout the course of treatment, and indicate the...